Extraction of chlorophyll from pandan leaves using ethanol and mass transfer study

Meilana Dharma Putra, Agus Darmawan, Ilham Wahdini, Ahmed E. Abasaeed


Green pigments are used in many industrial branches including food, drinks, soap and cosmetics. Chlorophyll can substitute synthetic dyes which may affect health. Chlorophyll can be extracted from pandan leaves; the pan­dan crop grows in many tropical areas. The effects of temperature, 30–70 °C and agitation speed, 100–400 rpm on chlorophyll extraction from pandan leaves, using ethanol and the evaluation of mass transfer coefficient, using dim­ensionless analysis were investigated. The optimal conditions of extraction was obtained at 60 °C and 300 rpm; the chlorophyll concentration was 107.1 mg L-1. The volumetric mass transfer coefficient increased with the temperature and agitation speed. Determination of volumetric mass transfer coefficient and dim­ensionless correlations are useful for further process development or industrial applications.


green pigments; chlorophyll; solvent extraction; dimensionless; temperature; agitation

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A. M. Humphrey, J. Food Sci. 69 (2004) C422

G. Özkan, S. E. Bilek, Food Chem. 176 (2015) 152

R. E. Kirk, D. F. Othmer, M. Grayson, Encyclopedia of Chemical Technology, The Interscience Encyclopedia Inc., New York, 1998

S. M. Hung, B. D. Hsu, S. Lee, J. Food Eng. 128 (2014) 17

U. M. Lanfer-Marquez, R. M. C. Barros, P. Sinnecker, Food Res. Int. 38 (2005) 885

M. G. Ferruzzi, J. Blakeslee, Nutr. Res. 27 (2007) 1

B.-E. V. Wyk, Food Plants of the World, Timber Press, Inc, Portland, OR, 2005

F. Salisbury, C. W. Ross, Plant Physiology, Brooks/Cole, Boston, MA, 1991

S. W. Jeffrey, R. F. C. Mantoura, S. W. Wright, Phytoplankton Pigments in Oceano-graphy: Guidelines to Modern Methods, UNESCO, Paris, 1997

D. Simon, S. Helliwell, Water Res. 32 (1998) 2220

A. Hosikian, S. Lim, R. Halim, M. K. Danquah, Int. J. Chem. Eng. 2010 (2010) 391632

R. F. C. Mantoura, C. A. Llewellyn, Anal. Chim. Acta 151 (1983) 297

M. D. Macías-Sánchez, C. Mantell, M. Rodríguez, E. Martínez de la Ossa, L. M. Lubián, O. Montero, Talanta 77 (2009) 948

J. E. Cacace, G. Mazza, J. Food Eng. 59 (2003) 379

T. C. Frank, J. R. Downey, S. K. Gupta, Chem. Eng. Prog. 95 (1999) 41

M. H. Abdel-Aziz, M. Bassyouni, I. A. S. Mansour, A. Nagi, J. Ind. Eng. Chem. 20 (2014) 2650

J. Saien, V. Moradi, J. Ind. Eng. Chem. 18 (2012) 1293

L. Schrive, G. Lumia, F. Pujol, N. Boussetta, Eur. Food Res. Technol. 239 (2014) 707

N. Wasmund, I. Topp, D. Schories, Oceanologia 48 (2006) 125

M. T. Fernández-Ponce, B. R. Parjikolaei, H. N. Lari, L. Casas, C. Mantell, E. J. Martínez de la Ossa, Chem. Eng. J. 299 (2016) 420

S. Sulaiman, A. R. Abdul Aziz, M. Kheireddine Aroua, J. Food Eng. 114 (2013) 228

S. Sayyar, Z. Z. Abidin, R. Yunus, A. Muhammad, Am. J. Appl. Sci. 6 (2009) 1390

C. J. Geankoplis, Transport Processes and Separation Process Principles, Prentice Hall, Upper Saddle River, NJ, 2003

J. Gross, Pigments in Vegetables, Van Nostrand Reinhold, New York, 1991

K. Zhang, P. Sun, H. Liu, S. Shang, J. Song, D. Wang, Carbohydr. Polym. 138 (2016) 237

H.-M. Liu, F.-Y. Wang, Y.-L. Liu, Food Chem. 202 (2016) 104.

DOI: http://dx.doi.org/10.2298/JSC161203038P


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