Effective valorization of barley bran for simultaneouscellulase and β-amylase production by Paenibacilluschitinolyticus CKS1: Statistical optimization and enzymes application

Katarina R. Mihajlovski, Slađana Z. Davidović, Đorđe N. Veljović, Milica B. Carević, Vesna M. Lazić, Suzana I. Dimitrijević-Branković

Abstract


The agricultural raw industry generates large amounts of annually by products, which create disposal problems. Up to now, there are no reported papers about simultaneous production of cellulase and β-amylase on these raw materials using Paenibacillussp., which would reduce the costs.  Thus, in this paper simultaneous cellulase (CMC-ase and Avicelase) and β-amylase production using barley bran by natural isolate Paenibacilluschitinolyticus CKS1 and potential enzymes application in the hydrolysis process was studied. Response Surface Methodology was used for obtaining the maximum enzyme activity (CMCase0.405 U ml-1, Avicelase 0.433 U ml-1 and β-amylase 1.594U ml-1). Scanning electron microscopy showed degradation of lignocellulosic-starch structure of barley bran after fermentation.The CKS1 bacterial supernatant, which contains cellulases and β-amylase could hydrolyze cotton fibers and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibers and barley bran, glucose (0.117 g gmat-1) and maltose (0.347 g gmat-1), were quantified by high performance liquid chromatography (HPLC).  Produced enzymes can be used for hydrolysis of cotton fabric and barley bran, to glucose and maltose, respectively. Application of simultaneous enzymes production using agricultural by product is economically and environmentally accepted and also valuable biotechnological products, as glucose and maltose were obtained in this investigation.


Keywords


agricultural by product; P. chitinolyticus CKS1; enzymes; process optimization; degradation; hydrolysis products

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DOI: http://dx.doi.org/10.2298/JSC170514092M

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