Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase

Xin Wang, Shiyu Sun, Zijun Ni, Zhaoxing Li, Jia Bao

Abstract


The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase has been reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 (v/v) in soil and PAH solution, subsequently the immobilization methods of adsorption-crosslink composite was applied to immobilize the laccase derived from fungi onto nylon net and chitosan, respectively. These two kinds of immobilized laccase were used in the degradation of pyrene (Pyr) and benzopyrene (BaP), and their degradation efficiencies under different temperature and pH conditions were also investigated. Consequently, the optimal laboratory experiment parameters were described as follows: Firstly, compared with free laccase, the degradation rates of Pyr and BaP by immobilized laccase increased by around 10%-30%. Secondly, the degradation efficiency of chitosan as a carrier of immobilized laccase was much better than that of nylon net, as a carrier of the immobilized laccase. Finally, when the temperature was set at 40℃, and pH was set as 4, the degradation efficiency of that immobilized laccase achieved the best.


Keywords


immobilized laccase; polycyclic aromatic hydrocarbons (PAHs); soil remediation; nylon net; chitosan.

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DOI: http://dx.doi.org/10.2298/JSC171004022W

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