A novel compound isolated from Sclerochloa dura has anti-inflammatory effects

Syed Majid Bukhari; Astrid Jullumstrø Feuerherm; Hanna Maja Tunset; Stian Moe Isaksen; Mari Sæther; Thor Håkon Thvedt; Susana Villa Gonzalez; Rudolf Schmid; Anders Brunsvik; Erik Fuglset; Bojan Zlatković; Berit Johansen; Nebojša Simić

doi:10.2298/JSC150119055B

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Published: Feb 5, 2016

DOI: https://doi.org/10.2298/JSC150119055B

Keywords:

arachidonic acid anti-inflammatory activity PLA2 inhibitor Sephadex LH-20 HPLC Cell line SW982.

Syed Majid Bukhari

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim, Norway and Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad-22060, KPK

Astrid Jullumstrø Feuerherm

Department of Biology, Norwegian University of Sciences and Technology, 7491 Trondheim

Hanna Maja Tunset

Department of Biology, Norwegian University of Sciences and Technology, 7491 Trondheim

Stian Moe Isaksen

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim,

Mari Sæther

Department of Biology, Norwegian University of Sciences and Technology, 7491 Trondheim

Thor Håkon Thvedt

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim,

Susana Villa Gonzalez

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim,

Rudolf Schmid

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim,

Anders Brunsvik

SINTEF Materials and Chemistry, Department of Mass Spectrometry, Sem Sælands vei 2a, 7465 Trondheim

Erik Fuglset

Technology transfer office, Norwegian University of Sciences and Technology, 7491 Trondheim

Bojan Zlatković

Department of Biology, Norwegian University of Sciences and Technology, 7491 Trondheim

Berit Johansen

Department of Biology, Norwegian University of Sciences and Technology, 7491 Trondheim

Nebojša Simić

Department of Chemistry, Norwegian University of Sciences and Technology, 7491 Trondheim

Abstract

The activation of PLA₂ by means of pro-inflammatory cytokines results in the subsequent release of arachidonic acid (AA) and generates eicosanoids, which further propagate inflammation. By 6the cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to eicosanoids, the non-steroidal anti-inflammatory drugs are effective in relieving the pain and discomfort of inflammation. By using AA release assay as a guide for biological and anti-inflammatory activity, novel compound 1-O-(3-O-linolenoyl-6-deoxy-6-sulfo-α-D-glucopyranosyl)-glycerol (1) together with five known compounds isovitexin, byzantionoside B, tricin 4-O-(erythro-β-guaiacylglyceryl) ether 7-O-β-glucopyranoside, 1-O-feruloyl glycerol and tricin 7-glucoside were isolated from the methanol extract of the aerial parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography, preparative HPLC and semi-preparative HPLC). All isolated compounds were identified using spectroscopic NMR spectroscopy and MS spectrometry. Novel compound (1) was found to be an effective inhibitor of AA release with an IC₅₀value of 0.09 ± 0.03 mg mL^-1.

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How to Cite

[1]

S. M. Bukhari, “A novel compound isolated from Sclerochloa dura has anti-inflammatory effects”, J. Serb. Chem. Soc., vol. 81, no. 1, pp. 1–12, Feb. 2016.

Issue

Vol. 81 No. 1 (2016)

Section

Organic Chemistry

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution license 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.