Degradation of chlorpyrifos in contaminated soil by immobilized laccase

Abstract

In the present study, the embedding-crosslinking method was used to immobilize fungal laccase, and to determine the suitable conditions for immo­bilization by measuring various activities of the immobilized laccase. In addi­tion, the immobilized laccase was further employed to repair chlorpyrifos-con­taminated soil, and then the degradation rates of chlorpyrifos were measured under different conditions. Based on the results, the appropriate conditions for the method of embedding-crosslinking were found to be as follows: concen­tra­tion of sodium alginate – 3 %, concentration of glutaraldehyde – 1 %, cross­link­ing time – 6h, volume of crude laccase – 60 ml, and immobilization time – 4 h. After 48 h, the degradation rate of chlorpyrifos in soil could reach at least 70 % by the use of immobilized laccase that was prepared by the method of embedding-crosslinking under different environmental conditions.