Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals

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Simona Oancea
Mirabela Perju
Horea Olosutean


Peony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the develop­ment of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extraction of peony phenolics were first investigated by comparing to conventional extract­ion and testing the data fit by mathematical models (linear, two factor inter­actions, quadratic) followed by investigation of cellulase pre-treatment com­bined with ultrasonication. High amounts of flavonoids, phenolics and tannins were extracted in 30 min under ultrasound conditions of 150 W, 40 kHz, 50 % amplitude, in 70 % ethanol with 50/1 solvent/solid ratio. A further cellulase pre-treatment at 40 °C for different incubation times combined with ultrasonic­ation facilitated the extraction of anthocyanins (720.48 mg/100 g DW), phen­olics (3985.50 mg/100 g DW), flavonoids (2369.28 mg/100 g DW) and tannins (8917.81 mg/100 g DW). Good antioxidant activities by FRAP (350.03 mg ascorbic acid/100 g DW) and DPPH (inhibition values >80 %) were registered showing great potential for developing high-value bioingredients. The FTIR analysis revealed the presence of characteristic functional groups. The hereby-combined extraction methods could be extended to other peony species.


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S. Oancea, M. Perju, and H. Olosutean, “Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals”, J. Serb. Chem. Soc., vol. 85, no. 7, pp. 845–856, Jul. 2020.
Biochemistry & Biotechnology


R. N. Cavalcanti, T. Forster-Carneiro, M. T. M. S. Gomes, M. A. Rostagno, J. M. Prado, M. A A. Meireles, Uses and applications of extracts from natural sources, RSC Publishing, Cambridge, 2013, pp. 1–57 (

V. V. Teplova, E. P. Isakova, O. I. Klein, D. I. Dergachova, N. N. Gesler, Y. I. Deryabina, Appl. Biochem. Microbiol. 54 (2018) 221 (

M. M. Jucá, C. F. M. S. Filho, J. C. de Almeida, D. D. S. Mesquita, J. R. M. Barriga, K. C. F. Dias, T. M. Barbosa, L. C. Vasconcelos, L. K. A. M. Leal, J. E. Ribeiro, S. M. M. Vasconcelos, Nat. Prod. Res. 16 (2018) 1

F. Ahmad, N. Tabassum, S. Rasool, Int. Res. J. Pharm. 3 (2012) 85 (

L. C. Soare, I. Deliu, I. Iosub, C.-M. Dobrescu, M. Ferdes, Curr. Trends Nat. Sci. 1 (2012) 79 (

L. C. Soare, M. Ferdes, S. Stefanov, Z. Denkova, R. Nicolova, P. Denev, C. Ungureanu, Rev Chim.-Bucharest 63 (2012) 432 (

F. Toma, E. Selaru, M. I. Georgescu, S. Petra, I. M. Padure, D. Zamfir-Vasca, O. Venat, AgroLife Sci. J. 2 (2013) 103 (

N. Jia, Q.-Y. Shu, D.-H. Wang, L.-S. Wang, Z.-A. Liu, H.-X. Ren, Y.-J. Xu, D.-K. Tian, K. M. Tilt, J. Am. Soc. Hortic. Sci. 133 (2008) 418 (

K. Ghafoor, Y. H. Choi, J. Y. Jeon, I. H. Jo, J. Agric. Food Chem. 57 (2009) 4988 (

M. Puri, D. Sharma, C. J. Barrow, Trends Biotechnol. 30 (2012) 37

A. S. N. Formagio, D. D. Ramos, M. C. Vieira, S. R. Ramalho, M. M. Silva, N. A. H. Zárate, M. A. Foglio, J. E. Carvalho, Braz. J. Biol. 75 (2015) 69 (

C. Grajeda-Iglesias, E. Salas, N. Barouh, B. Baréa, M. C. Figueroa-Espinoza, Food Chem. 230 (2017) 189 (

L. Lotfi, A. Kalbasi-Ashtari, M. Hamedi, F. Ghorbani, J. Food Drug Anal. 12 (2015) 210 (

V. L. Singleton, J. A. Rossi Jr., Am. J. Enol. Vitic. 16 (1965) 144 (

S. Kumar, D. Kumar, Manjusha, K. Saroha, N. Singh, B. Vashishta, Acta Pharmaceut. 58 (2008) 215 (

M. L Price, S. Van Scoyoc, L. G. Butler, J. Agr. Food Chem. 26 (1987) 1214 (

M. M. Giusti, R. E. Wrolstad, Current protocols, Wiley, New York, 2001 (

F. F. Benzie, J. J. Strain, Anal. Biochem. 239 (1996) 70 (

W. Brand-Williams, M. E. Cuvelier, C. Berset, Lebensm. Wiss. Technol. 28 (1995) 25

W. Kruskal, W. A. Wallis, J. Am. Stat. Assoc. 47 (1952) 583 (

K. Rajbhar, H. Dawda, U. Mukundan, Sci. Rev. Chem. Commun. 5 (2015) 1 (

G.-L. Chen, S.-G. Chen, Y.-Q. Xie, F. Chen, Y.-Y. Zhao, C.-X. Luo, Y.-Q. Gao, J. Funct. Food 17 (2015) 243 (

W. Li, S. Yang, H. Cui, Y. Hua, J. Tao, C. Zhou, Emir. J. Food Agric. 29 (2017) 518 (

Y. Wu, M.-R. Wei, D.-Q. Zhao, J. Tao, J. Integr. Agr. 15 (2016) 2023 (

V. Mancas, S. Amariei, Food Environ. Saf. 15 (2016) 95 (

F. Shahidi, J. Yeo, Molecules 21 (2016) 1 (

L. Liu, W. Wen, R. Zhang, Z. Wei, Y. Deng, J. Xiao, M. Zhang, Food Chem. 214 (2017) 1 (

C. Renard, A. Watrelot, C. Le Bourvellec, Trends Food Sci. Tech. 60 (2017) 43

V. G. Yachmenev, B. D. Condon, A. H. Lambert, Intensification of enzymatic reactions in heterogeneous systems by low intensity, uniform sonication: new road to "green chem¬istry", American Chemical Society, Washington DC, 2007 (ISBN-13: 978-0841274068)

S. J. Marathe, S. B. Jadhav, S. B. Bankar, R. S. Singhal, Enzyme-assisted extraction of bioactives, Springer Internationl Publishing, Cham, 2017, pp. 171–201 (

Y.-H. Lee, E.-K. Hwang, H.-D. Kim, Materials 2 (2009) 10 (