Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)

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Sandra Sretko Bulatović
Nenad Marić
Tatjana Šolević Knudsen
Jelena Avdalović
Mila Vladislav Ilić
Branimir Jovančićević
Miroslav M Vrvić


Due to their extensive use, petroleum hydrocarbons are among the most common groundwater contaminants. Compared to the traditional methods of physical pumping of contamination from the aquifer and subsequent treat­ment (i.e., pump and treat), bioremediation is an economically cost-effective technology. The aim of this remediation approach is to transform biologically contaminants, most often by microbiological activity, into non-toxic com­pounds. More precisely, it is an active remediation process that involves bio­stimulation (increase of aquifer oxygenation, addition of nutrients) and/or bio­augmentation (injection of a concentrated and specialized population of micro­organisms). Using both biostimulation and bioaugmentation, enhanced in situ groundwater bioremediation was applied at a hydrocarbon-contaminated site in Belgrade. The bioremediation treatment, applied over twelve months, was highly efficient in reducing the concentrations of total petroleum hydrocarbon (TPH) to acceptable levels. The concentration of TPH in the piezometer P-5 was reduced by 98.55 %, in the piezometer P-6 by 98.30 % and in the piezo­meter P-7 by 98.09 %. These results provided strong evidence on the potential of this remediation approach to overcome site-limiting factors and enhance microbiological activity in order to reduce groundwater contamination.


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How to Cite
S. S. Bulatović, “Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)”, J. Serb. Chem. Soc., vol. 85, no. 8, pp. 1067–1081, Aug. 2020.
Environmental Chemistry


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