@article{Zavišić_Petričević_Ristić_Rikalović_Jovanović-Lješković_Begović_Strahinić_2019, place={Belgrade, Serbia}, title={Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns}, volume={84}, url={https://www.shd-pub.org.rs/index.php/JSCS/article/view/7562}, DOI={10.2298/JSC181105015Z}, abstractNote={<p class="AbstractChar">The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on <em>16S rRNA</em> gene sequencing. In order to evaluate its probiotic properties <em>in vitro</em> tests were performed: the survival in simulated gastroint­es­tinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tole­rance and bacterial translocation<em> in vivo</em>, as well as hemolytic activity <em>in vitro</em>. The isolate G-4, identified as <em>Lactobacillus fermentum</em>, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL-1 units, respectively), moderate adhesion to hexa­decane (39±2.1 %), and antimicrobial activity against <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, <em>Salmonella enterica </em>subsp<em>. enterica </em>serotype Abony and <em>Clostridium sporogenes</em>, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.</p>}, number={4}, journal={Journal of the Serbian Chemical Society}, author={Zavišić, Gordana Nikola and Petričević, Saša M and Ristić, Slavica M and Rikalović, Milena G and Jovanović-Lješković, Nataša M and Begović, Jelena M and Strahinić, Ivana D}, year={2019}, month={Apr.}, pages={365–376} }