Production of biosolvents and acids by salinity-adapted strain of Clostridium acetobutylicum: Effects of salt and molasses concentrations

Reza Zabihi, Dariush Mowla, Gholamreza Karimi


In this study, growth of Clostridium acetobutylicum is evaluated in Clostridium basal medium (CBM) containing 0.001, 0.5, 1, 2 and 4 % salt concentrations. Although the strain was sensitive to salinity of more than 2 %, the adapted strain is shown to grow even at 6 % salinity. The results state adverse effects of salinity on bacterial growth and bioproducts such as butanol and butyric acid, whereas produced acetone is increased by salinity in CBM. In addition, glucose of CBM is substituted by sugar beet molasses, due to its lower price and higher accessibility. Therefore, molasses-based mediums (MBM) at different molasses concentrations are examined to assess the effect of molasses concentration on the adapted strain at low salinity. The results show that 4 and 6 % (w/v) molasses concentrations are optimum concentrations for bacterial growth and its bioproducts production at low salinity. Finally, the simultaneous effects of salinity and molasses concentration on the adapted strain are investigated. For this purpose, molasses-based mediums (MBM) containing 2, 3 and 4% (w/v) molasses concentration at 4 % salinity are considered. The results demonstrate that the increase in the molasses concentration raises the production of both butyric acid and acetone.  


Clostridium acetobutylicum; adapted strain; salinity; sugar beet molasses; bioproducts

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