The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment

Aneta V. Buntić, Olivera S. Stajković-Srbinović, Dušica I. Delić, Suzana I. Dimitrijević-Branković, Marija D. Milić


Agricultural by-products are generated in large amounts in various ind­ustries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and аvicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g-1 liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g-1 and avicelase of 1.417 U g-1) were achieved using of the strain CKS1.


lignocellulosic waste; microwave-assisted extraction; solid-state fermentation; CMCase and Avicelase activity

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