Effects of Hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro

Biljana Davidović-Plavšić, Nataša Lukić, Aleksandra Nikolić-Kokić, Biljana Kukavica

Abstract


The aim of this work was to investigate the effect of the herbicide, commercial formulation Hemazin SC 500 containing terbuthylazine as an active compound on isoenzyme patterns and activities of CuZn superoxide dismutase (SOD1) and catalase (CAT), as well as on glutathione-S-transferase (GST) activity in human erythrocytes in vitro. The human erythrocytes were treated with Hemazin SC 500 in broad terbuthylazine concentrations (37 nmol/L - 37 mmol/L) for 1 and 3 h at the temperature of 37 ºC. Native electrophoresis in control and treated samples revealed two SOD1 and one CAT isoform. Treatment did not affect SOD1 and CAT isoenzyme profile, but induced a change in their activities. Terbuthylazine at lower concentration has induced a significant increase of total SOD1 activity and decreased GST activity in samples incubated for 1 and 3 h. On the other hand, the highest increase of CAT activity was observed in the sample treated for 1 h with higher concentration of terbuthylazine. Hemazin SC 500 containing terbuthylazine induces changes in the erythrocyte antioxidative system whereby the response of individual enzymatic antioxidants depends on the concentration of the pesticide and the incubation time.


Keywords


herbicide; hemolysate; SOD1; catalase; glutathione-S-transferase

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DOI: https://doi.org/10.2298/JSC181011115D

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