Headspace gas chromatography–mass spectrometry method for the determination of total cyanide concentration in water and post-mortem blood samples

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Orhan Destanoğlu
İsmail Ateş


In this study, we aimed to develop a headspace gas chromatography–
–mass spectrometry method for determining the total cyanide concentration in the forensic evidences. Total cyanide content of the samples was calculated based on the hydrogen cyanide gas concentration evaporated from the liquid sample in the headspace vial. Hexacyanoferrate(II) was used for the optimiz­ation of headspace oven temperature. We have found that iron–cyanide bonds were completely degraded after 0.2 mL of the sample was treated with 1 mL of 1 M sulfuric acid under the optimized headspace conditions where the tempera­ture and the heating time were 120 °C and 12.5 min, respectively. Satisfactory recovery results for both aqueous and blood samples were obtained. The method was linear in the range 0.05–10 µg mL-1 of cyanide which was a suit­able range for toxicological investigations. The proposed method was validated and applied to the post-mortem blood samples, drinking waters, and the other forensic evi­dences. The proposed method can easily be performed not only in the forensic laboratories, but in the related laboratories where the total cyanide analysis is a critical issue.


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O. Destanoğlu and İsmail Ateş, “Headspace gas chromatography–mass spectrometry method for the determination of total cyanide concentration in water and post-mortem blood samples”, J. Serb. Chem. Soc., vol. 86, no. 1, pp. 77–90, Jan. 2021.
Analytical Chemistry


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