HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua Scientific paper

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Jovana Stanković Jeremić
Dejan Gođevac
Stefan Ivanović
Katarina Simić
Antoaneta Trendafilova
Milica Aćimović
Slobodan Milosavljević


The application of high performance thin layer chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is des­cribed. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate ana­lysis. Principal component analysis and orthogonal partial least squares dis­criminant analysis confirmed the differentiation of samples belonging to veget­ative phase, flowering stage, and seed formation stage of the plant develop­ment. The obtained results showed that the HPTLC-based metabolomics appro­ach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to gas chromatography–mass spec­trometry and nuclear magnetic resonance-based metabolomics.


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How to Cite
J. Stanković Jeremić, “HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua: Scientific paper”, J. Serb. Chem. Soc., vol. 87, no. 11, pp. 1237–1244, Oct. 2022.
Organic Chemistry


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